Umbilical Vein Endothelial Cell Antioxidant Evaluation Service
Endothelial cells are single-layer flat cells lining the inner surface of blood vessels and lymphatic vessels. They have a variety of physiological functions. As the first barrier between tissue and blood, endothelial cells are one of the first cells to feel hypoxia. Free radicals, reactive oxygen species, lipid peroxides, and ho can cause endothelial cell damage. Studies have proved that there is a causal relationship between the formation of oxygen free radicals and the occurrence of cardiovascular diseases. People have paid extensive attention to the damage of oxygen free radicals to the cardiovascular system. Antioxidants can reduce or eliminate free radicals, and prevent or reduce the damage caused by free radical oxidative stress. A large number of anti-free radical drugs or antioxidants have been developed, and even free radicals have been used as auxiliary indicators for disease diagnosis.
Creative Bioarray provides services for evaluating the protective effect of antioxidants on oxidative damage of vascular endothelial cells in vitro. Based on the established in vitro vascular endothelial cell oxidative stress model, we can simultaneously provide antioxidant administration experiments, pharmacy pharmacology, efficacy evaluation, and pharmacokinetic analysis. The purpose of this service is to promote the screening and development of antioxidants and anti-free radicals in the circulatory system.
Service Case
Procyanidins (PC) are powerful antioxidants, which were initially used as an effective drug to protect the liver. With the development of the clinical application, PC has shown a significant protective effect on the cardiovascular system.
Our customers want to add PC to their products, so we need to explore whether proanthocyanidins have a protective effect on endothelial cell damage.
Preparation of experimental scheme
H2O2 is the active oxygen produced by the body, which can be decomposed into a series of complex products under peroxide conditions, such as oxygen-free radicals. Oxygen-free radicals can cause cell damage through the peroxidation of polyunsaturated fatty acids in biofilm, cause lipid peroxidation in cells, destroy membrane structure, increase membrane permeability, leading to tissue damage, and finally form atherosclerotic plaque.
We first used hydrogen peroxide to induce oxidative stress in human umbilical vein endothelial cells. In vitro cell, culture method was used to determine the cell proliferation activity and cell damage markers before and after PC intervention, to help customers explore the anti-cell damage effect of procyanidins and its possible mechanism.
Grouping
Human umbilical vein endothelial cells cultured in vitro were used for experiments after passage. The experiments were divided into three groups:
Routine culture of the control group
Hydrogen peroxide injury group
Drug intervention
Assay services
- The effect of procyanidins on the activity of H2O2 injured vascular endothelium was detected by MTT colorimetry
- The amount of MDA and LDH released into the culture medium was measured to judge the cell damage
- Cell superoxide dismutase activity was measured by the xanthine oxidase method
- Apoptosis assay
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
All services and products are for scientific use only, not for medical use！
