Umbilical Vein Endothelial Cell Oxidative Stress Modeling Service
Oxidative stress means that the balance between oxidants and antioxidants is broken and reactive oxygen species (ROS) are overexpressed to induce apoptosis, leading to endothelial cell damage. Vascular endothelial cells not only play an important barrier role, but also release a variety of bioactive substances such as prostacyclin, endothelium-derived relaxing factor, and endothelin, which play an important role in regulating vascular tension and maintaining the flow state of blood in blood vessels. When endothelial cells are damaged, their barrier function, endocrine function, and anticoagulation process may be impaired, which becomes the initial link of atherosclerosis.
There are mainly two kinds of oxidative stress models: animal model and cell model. The cell model has perfect metabolic function and electrophysiological characteristics avoid the interaction between different cells in the whole body, has strong specificity and controllability, and is easy to repeat.
Human umbilical vein endothelial cells (HUVECs) cultured in vitro have various functions of endothelial cells and can be used as a tool to study cardiovascular diseases. Creative Bioarray established an in vitro vascular endothelial cell oxidative damage model for our customers to observe its oxidative damage effect on vascular endothelial cells, and to lay a foundation for further oxidative damage inhibition experiments. There are two kinds of stress commonly used by us, hydrogen peroxide and oxidized low-density lipoprotein (ox-LDL).
H2O2 Induction
This is a common stressor. Hydrogen peroxide (H2O2) can easily penetrate the cell membrane and form highly active free radicals with intracellular iron ions. It is the main component causing vascular damage. H2O2 is easy to obtain and stable in nature. It is an important tool for studying oxidative damage.
Our services include
- Establishment and sale of stress cell model
- Modeling oxidative stress with a customer is given cell lines
a. After the given cells were resuscitated and cultured, multiple control groups were established to determine the optimal dose and treatment time for H2O2 modeling. The morphology of H2O2 treated cells was observed by an inverted phase-contrast microscope.
b. The cell survival rate was detected by the CCK-8 method.
c. Apoptosis was detected by flow cytometry TUNEL method.
d. Intracellular ROS detection.
ox-LDL Induction
Under the action of oxygen-free radicals, LDL is oxidized. ox-LDL can induce the damage of vascular endothelial cells (ECV), and make ECV degenerate, necrosis, and fall off. VEC with oxidative damage can promote the proliferation of vascular smooth muscle cells, platelet aggregation, and adhesion of monocytes to endothelial cells, and promote macrophages to ingest LDL and enter the vascular wall, thus forming foam cells, leading to the formation of atherosclerosis.
Our services include
- Establishment and sale of stress cell model
- Modeling oxidative stress with a customer is given cell lines
Ox-LDL containing different concentrations of MDA was incubated with cultured human umbilical vein endothelial cells in vitro to observe its oxidative damage effect on vascular endothelial cells, and to establish an in vitro vascular endothelial cell oxidative damage model, which laid a foundation for further oxidative damage inhibition experiments.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
All services and products are for scientific use only, not for medical use！
