Fluoride Oxidase Modeling Service for Osteoblast
Fig. 1 Bone remodeling cycle (Wittkowske, Reilly, Lacroix & Perrault, 2016)
Osteoblasts are very sensitive to fluoride and play a key role in the occurrence and development of skeletal fluorosis. Therefore, in recent years, people pay more and more attention to the deep exploration of the mechanism of fluoride on osteoblasts. In the study of the pathogenesis of chronic fluorosis, free radicals and oxidative stress are also one of the hotspots in recent years.
Creative Bioarray established the oxidative stress model of osteoblast cells by stimulating it with NaF.
We can also adjust the modeling process according to the specific needs of customers, provide administration experiment, pharmacology, efficacy evaluation and pharmacokinetic analysis, and assist customers to study osteoblast stress and / or pathological significance, which has important theoretical significance for clarifying orthopaedic related diseases.
Technology Principle
Fluorosis will weaken the body's ability to resist free radicals, reduce antioxidants such as glutathione (GSH), glutathione peroxidase (GPX), superoxide dismutase (SOD) and ascorbic acid, and increase free radicals in the body.
What We Do
Osteoblast culture and modeling
The third-generation osteoblasts were taken from the calvaria of suckling mice and cultured with NaF at appropriate concentration and time to induce cell stress.
Modeling quality evaluation system
Cell viability was assessed by MTT assay. The apoptosis rate was analyzed by flow cytometry and TUNEL assay. Protein levels were detected by Western blot.
Using the established osteoblast oxidative stress model, we can help customers explore the antioxidant effect of antioxidants in cells and provide corresponding experimental reports. The changes of intracellular lipid peroxidation products (MDA) and antioxidant enzymes (SOD, GPX) activities before and after the action of antioxidants were observed.
Model Applications
- It can be used for the role of fluorosis in the occurrence and development of oxidative stress.
- The model can be used for subsequent antioxidant screening experiments.
- The model can also be used as the endoplasmic reticulum oxidative stress model of osteoblasts.
- The model can be used to study the effects of oxidative stress on the morphology and proliferation of human osteoblast cells, and provide a theoretical basis for further studying the mechanism of oxidative stress in chronic bone resorption diseases such as periodontitis inflammation.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you at the first time.
Reference
- Wittkowske, C., Reilly, G., Lacroix, D., & Perrault, C. (2016). In Vitro Bone Cell Models: Impact of Fluid Shear Stress on Bone Formation. Frontiers In Bioengineering and Biotechnology, 4.
All services and products are for scientific use only, not for medical use！
