H2O2 Oxidase Modeling Service for Osteoblast
Osteoporosis is a common systemic bone disease, which is characterized by decreased bone mass and degeneration of bone tissue structure. Recent evidence shows that oxidative stress is caused by the imbalance of oxidants and antioxidants in favor of the former, which is the main factor in the development and progress of osteoporosis.
Fig. 1 ROS signaling cascades regulate osteoclast and osteoblast (Tao et al., 2020)
Osteoblast apoptosis plays an important role in bone development and maintenance, and contributes to osteoporosis, glucocorticoid excess and aging caused by sex steroid deficiency to a certain extent. Many studies have shown that oxidative stress continues to increase under these pathological conditions, which may help to inhibit osteoblast differentiation and proliferation or induce apoptosis.
Creative Bioarray established the oxidative stress model of human osteoblast like cells by stimulating it with H2O2.
What We Do
Osteoblast culture and modeling of the given strains
Primary cultured human osteoblasts were established by standard technology and identified by bone nodules and alkaline phosphatase. The cells were cultured in the incubator. According to the changes of cell growth density and medium pH, the medium is changed every 2 ~ 3 day. The experiment was carried out when the cells grew to the logarithmic growth stage.
We have found the best concentration and time of H2O2 induction. However, if you choose other cell lines, we will conduct a small-scale grouping experiment before modeling to determine the best modeling conditions.
Modeling quality evaluation system
Cell viability was assessed by MTT assay. The apoptosis rate was analyzed by flow cytometry and TUNEL assay. Mitochondrial ROS and membrane potential were measured by fluorescence microscope. Mitochondrial respiratory enzyme activity was measured using a spectrophotometer. Protein levels were detected by Western blot.
Drug administration and evaluation services
We can also adjust the modeling process according to the specific needs of customers, provide administration experiment, pharmacology, efficacy evaluation and pharmacokinetic analysis, and assist customers to study osteoblast stress and / or pathological significance, which has important theoretical significance for clarifying orthopaedic related diseases.
Using the established osteoblast oxidative stress model, we can help customers explore the antioxidant effect of antioxidants in cells and provide corresponding experimental reports.
Model Application
The model can be used to study the effects of oxidative stress on the morphology and proliferation of human osteoblast like cells, and provide a theoretical basis for further studying the mechanism of oxidative stress in chronic bone resorption diseases such as periodontitis inflammation.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you at the first time.
Reference
- Tao, H., Ge, G., Liang, X., Zhang, W., Sun, H., Li, M., & Geng, D. (2020). ROS signaling cascades: dual regulations for osteoclast and osteoblast. Acta Biochimica Et Biophysica Sinica, 52(10), 1055-1062.
All services and products are for scientific use only, not for medical use！
