Animal Oxidative Stress Modeling and Evaluation Services
Stress refers to a series of nonspecific reactions when animals are stimulated by endogenous or exogenous stressors. When stressors stimulate animals, they will be excited by sympathetic nerves. The secretion of pituitary and adrenocortical hormones will increase, resulting in decreased feed intake, decreased feed conversion rate, enhanced catabolism, and decreased immune function. Stress has caused serious harm to animal health and production performance and has become the main factor restraining the development of large-scale intensive breeding. Environment, temperature, nutrition, feeding management, and genetic factors will lead to stress.
That is, when the body is stimulated by the external environment, ROS production increases or/and scavenging capacity decreases, causing flocculent disorder of the body's oxidation system and antioxidant system, resulting in the accumulation of free radicals in the body and causing damage. When cells are in a normal or abnormal physiological state, they can produce ROS substances with high activity, such as superoxide anion, hydrogen peroxide, and hydroxyl radical.
Creative Bioarray provides animal-based oxidative stress model establishment services for customers in need. We can select appropriate stressors and customize induction schemes for customers according to their needs.
Available Modeling Methods at Creative Bioarray
- Oxidative stress model induced by heavy metal vanadium -- suitable for rats or broilers
Vanadium salt can inhibit the activities of NADH dehydrogenase and succinate dehydrogenase in the mitochondrial respiratory chain, so that electrons are lost in the process of transferring to cytochrome c reductase, resulting in excessive production of ROS. Secondly, vanadium blocks its nuclear translocation by reducing the phosphorylation of transcription factor NF-E2 related factor 2 (Nrf2), thereby reducing the production of phase II detoxifying enzymes, causing ROS clearance barriers and ROS accumulation.
- Diquat induced oxidative stress model -- applicable to broilers and pigs
A bipyridyl herbicide, belonging to a moderately toxic compound; can convert molecular oxygen into superoxide anion free radicals, stimulate the production of cell-free radicals through the reduction of the periodic oxidation process, disorder the internal environment, and induce oxidative stress in animals.
- Oxidative stress model induced by other substances
Doxorubicin, vegetable oil, D-galactose in galactose synthase
Available Modeling Services at Creative Bioarray
Monitoring of Oxidative Stress in Experimental Animals
ROS is an inevitable by-product of aerobic energy metabolism. The body eliminates its potential threat to the body through a series of antioxidant mechanisms. Therefore, the detection of ROS level, antioxidant capacity, and the content of oxidative stress damage markers can be used to evaluate the occurrence and harm of oxidative stress.
It is a prerequisite to correctly evaluate the effect of stress on an animal's physiological function to select appropriate detection indicators and analysis methods. When investigating the changes in the oxidative stress state of animals after stress, we will select multiple indicators to comprehensively evaluate whether the body is in the state of oxidative stress by considering not only the changes of supporting oxidative effectors (such as lipid peroxidation) after stress but also the changes of antioxidant capacity of the body.
The research on the effect of stress on oxidative stress can not only provide experimental facts for researchers to develop drugs or methods to intervene against stress, but also provide enlightenment for exploring the mechanism of related organ diseases after stress.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
All services and products are for scientific use only, not for medical use！
