Antioxidant Activity In Vivo Evaluation
At Creative Bioarray, we have established a comprehensive antioxidant evaluation solution through in vitro, ex vivo cellular and in vivo assays. We have established simple, rapid and effective oxidation models in rats, mice, Drosophila and Cryptobacterium histolytica for the accurate evaluation of antioxidant activity of our subjects. The in vivo evaluation of antioxidants is a significant guide for their development and application in food, pharmaceutical, and daily chemical applications.
Evaluation of Antioxidant Activity in Drosophila
Drosophila is a eukaryotic multicellular organism whose physiological condition is basically similar to that of mammals, and it is more sensitive to experimental conditions, so it was used in our antioxidant experimental research.
Drosophila were fed with different doses of natural product extracts at the optimal UV irradiation dose, and then the antioxidant activity was evaluated by counting the lifespan of Drosophila, calculating the average lifespan, maximum lifespan and time to half death, and measuring the levels of CAT, SOD and other antioxidant enzymes, ROS and malondialdehyde (MAD).
Evaluation of Antioxidant Activity in Cryptobacterium Hidradenum
It is often used for lifespan-related analysis. We use nematodes for initial screening of candidates, and the active candidates are then validated and studied in depth in higher biological models such as mice and rats.
Evaluation of Antioxidant Activity in Rats and Mice
Our experiments for the evaluation of antioxidant activity in rats and mice mostly use aged animals or by inducing the production of peroxidative damage models, D-galactose models, radiation models, and bromodiphenyl models.
The antioxidant enzymes such as SOD, GSH-PX and CAT and the indices of induced peroxisomes such as MDA were measured in the subject samples against the aging model animals and compared with the blank control group to derive the antioxidant capacity of the subjects in the organism.
Please note that the antioxidant activity evaluation assay in rats and mice is suitable for the active compounds after in vitro screening, which has many complex assay indicators and long experimental period, and is not suitable for the pre-screening and bulk testing of antioxidant natural products.
Chicken embryo fibroblasts and skeletal cells are commonly used for the study of oxidative stress. They are stimulated by various inducers in vitro. The induction can cause changes in the main indicators of oxidative stress, such as increased levels of lipid peroxidation, decreased antioxidant enzyme activity, increased apoptotic protease activity, and ultimately increased cell mortality.
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