Antioxidant Activity In Vitro Evaluation
Antioxidants can play an effective defense role in reducing the damage of oxidation to the chemical structure of intracellular proteins, lipids, nucleic acids and other biological macromolecules. The establishment of simple, rapid and effective antioxidant models for the accurate evaluation of antioxidant activity of subjects is a significant guideline for their development and application in the food, pharmaceutical and daily chemical fields. Creative Bioarray provides objective and accurate in vitro antioxidant activity assays and cell-based in vitro antioxidant evaluation services.
Project Phase 1: In Vitro Activity Assay of Antioxidant Candidates
| Available assay methods |
Description |
| DPPH |
The antioxidant activity of the samples can be determined by the amount of DPPH·scavenged. |
| Pyrogallol autoxidation |
The change of absorbance value at a specific wavelength can be measured to obtain the autoxidation rate of o-triphenol after the addition of antioxidant, and then indirectly find the ability to scavenge O2·- |
| β-carotene bleaching assay |
It is mainly suitable for determining the antioxidant capacity of antioxidants in emulsified liposome systems. |
| Hydroxyl radical scavenging capacity |
Fenton reaction, Haber-Weiss reaction, Vc-Cu2+-H2O2 yeast polysaccharide system reaction and Vc-Cu-SO4-Cytc method, etc. |
| Chemiluminescence |
When antioxidants are present, the luminescence intensity decreases, and the antioxidant capacity is evaluated by calculating the intensity of antioxidant-inhibited luminescence. |
| ORAC |
The principle of this method is to terminate the free radical chain reaction by inhibiting the transfer reaction of hydrogen. |
| ABTS |
When ABTS·+ reacts with the antioxidant, colorless ABTS is produced, according to which the change in absorbance of the maximum absorption peak at 734 nm is detected to evaluate the antioxidant activity. |
| Ferric reducing antioxidant power |
The antioxidant reduced Fe3+-TPTZ to Fe2+-TPTZ with dark blue color at pH 3.6 and 37°C. |
Project Phase 2: Cell-Based in Vitro Antioxidant Evaluation
The activity of the antioxidant candidates can be screened for antioxidant capacity based on isolated cells after the screening in Project 1.
Advantages
Cultured cells are closer to the environment in the organism, and the antioxidant activity of natural products can be evaluated by cellular modeling experiments.
In vitro cell models are more biologically relevant than chemical assays, and faster and more economical than animal models and clinical studies.
Based on the cell model, we can further study the mechanism of distribution, uptake and transport, and metabolism of natural products in the cell.
Solution
We can select different model cells for our clients according to the application purpose of antioxidant substances.
| Research System |
Available Cell Models |
| Digestive System |
Intestinal Epithelial Cell, Hepatic Stellate Cell, Hepatoma Cell, Hepatocyte |
| Reproductive System |
Rat, Mouse, Human Ovarian Cell |
| Skeletal System |
Osteoblast, Chondrocyte |
| Circulatory System |
Umbilical Vein Endothelial Cell, Myocardial Cell |
| Nervous System |
Neural Cell, Neuroblastoma, Glioblastoma |
| Skin System |
Skin Fibroblast, Melanocyte |
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
All services and products are for scientific use only, not for medical use！
