Endoplasmic Reticulum Stress Model
Endoplasmic reticulum (ER) is a multifunctional organelle responsible for protein folding, modification, assembly and calcium storage in eukaryotic cells. Due to its huge and complex membrane structure and a large number of functional proteins attached to it, it has become the hub for regulating a variety of signal pathways. Only the correctly folded proteins in the ER can be transported to Golgi and become secretory proteins or membrane proteins. When cells are disturbed by different environmental factors, the folding of peptide chains in endoplasmic reticulum will be blocked, and a large number of misfolded proteins will gather, so that cells are in a state of stress, which is called endoplasmic reticulum stress (ERS). In recent years, great breakthroughs have been made in the research on the mechanism of endoplasmic reticulum stress leading to apoptosis and various diseases. Understanding the regulation mechanism of endoplasmic reticulum stress and UPR signal pathway is of great significance for studying new antiviral drugs, antioxidants, targeted therapeutic drugs and proposing new antiviral strategies.
Creative Bioarray already has a perfect cell culture technology platform. Based on this platform, we can provide customers with a variety of endoplasmic reticulum stress modeling services. At present, our research has induced endoplasmic reticulum stress on hepatocytes, fibroblasts, osteoblasts, liver cancer cells and cardiomyocytes by means of viruses (hepatitis B virus and rhinovirus) and tunicamycin.
In addition, we can also adjust the modeling process according to the specific needs of customers, provide administration experiment, pharmacology, efficacy evaluation and pharmacokinetic analysis, and assist customers to study the physiological and / or pathological significance of ERS.
Available Services and Models
Fig. 2 Endoplasmic Reticulum Stress Model (King & Wilson, 2020)
The regulatory mechanism and signal pathway of virus on endoplasmic reticulum stress are of great significance for the study of new antiviral drugs and new antiviral strategies. The company has established a plasmid vector that can stably overexpress hepatitis B virus HBx protein.
Rhinovirus (RhV) is the most important pathogen causing the common cold. We constructed the eukaryotic expression vector of rhinovirus nonstructural protein 2B. Endoplasmic reticulum stress model can be established in host cells by transfecting the expression vector.
The role of ERS in bone biology has attracted more and more attention. We used memycin, a recognized chemical that can lead to ERS in osteoblasts, as a stress source to simulate endoplasmic reticulum stress in human osteoblasts.
We induced human normal hepatocyte line (L02 cells) to construct human hepatocyte ERS model. The model is expected to provide experimental basis for further exploring the molecular mechanism of ERS mediated hepatocyte apoptosis related diseases.
Human ERS model was established by inducing neonatal rat cardiomyocytes with tunicamycin. The model is expected to provide a research basis for further exploring the pathogenesis of endoplasmic reticulum stress-induced cardiomyocyte apoptosis and related diseases.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you at the first time.
Reference
- King, A., & Wilson, J. (2020). Endoplasmic reticulum stress: an arising target for metal-based anticancer agents. Chemical Society Reviews, 49(22), 8113-8136.
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