Establishment of Stress Model by Biological Method
The cell model is one of the effective methods to screen and evaluate antioxidants and study the mechanism of oxidative stress injury. Creative Bioarray provides many types of cell stress modeling services. Based on the cell lines of nervous, urinary, immune, skeletal, circulatory, digestive, skin, and reproductive systems, we establish customized oxidative stress cell models and services for customers through chemical, physical, or biological stressors. Biological stressors mainly include genetic engineering technology and metabolites in vivo. We will artificially select disease-related stressors and establish in vitro simulated oxidative stress to reflect the action mechanism of antioxidants in the pathological state of the body.
Available Biological Stressor at Creative Bioarray
PCV2 is commonly used in the study of porcine virology and immunology. Macrophages play an important role in both innate and acquired immunity. We can treat given macrophages and other cell lines in vivo with porcine circovirus type 2 (PCV2) and establish oxidative stress models for customers.
HBV is a partially double-stranded DNA virus with an envelope. It is considered to cause ERS and is closely related to the occurrence and development of hepatocellular carcinoma. To further explore the relationship between hepatitis B virus and liver cancer, Creative Bioarray established a plasmid vector that can stably overexpress the HBx protein of hepatitis B virus.
RhV is the most important pathogen causing the common cold. The nonstructural protein 2B of rhinovirus has the characteristics of low relative molecular weight and a high degree of the hydrophobic region. Creative Bioarray constructed the eukaryotic expression vector of rhinovirus nonstructural protein 2B. By transfecting the expression vector, the endoplasmic reticulum stress model can be established in host cells.
Service Content
- Construction, amplification, and marketing of plasmids
- Host cell culture and plasmid transfection services
- Construction of cell stress model
- Modeling effect and subsequent index detection service
In addition, we can also adjust the modeling process according to the specific needs of customers, provide administration experiments, pharmacology, efficacy evaluation, and pharmacokinetic analysis, and assist customers to study the physiological and/or pathological significance of host cell stress, which has important theoretical significance for clarifying diseases.
Our Features
- Low cytotoxicity (ultracentrifugation purification process)
- No mycoplasma contamination
- There is no endotoxin, exogenous microorganisms, and acid host residue
- High virus culture titer
- Strong customer-centered after-sales service system
- Super large virus spot library
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
All services and products are for scientific use only, not for medical use！
