Hepatocyte Oxidative Stress Model
Digestive system is one of the eight systems of human body. As one of the most important digestive glands in the digestive system and even the whole organism, liver has always been a research hotspot. With the development of new cell technology, the research on oxidative stress of liver cells is gradually increasing.
Fig. 1 Hepatocyte Oxidative Stress (Tell, Vascotto & Tiribelli, 2013)
Oxidative stress is one of the important mechanisms of liver damage caused by liver diseases, including viral hepatitis, alcoholic liver disease and nonalcoholic fatty liver disease. Oxidative stress is usually caused by reactive oxygen species (ROS), which is induced by NAD (P) H oxidase, exogenous substances and mitochondrial metabolites of activated inflammatory cells in the liver, and cytochrome P450 oxidase family 2. Under pathological conditions, the production of ROS increases. When it exceeds the scavenging capacity of the antioxidant system, ROS attacks adjacent tissues and cells, causing oxidative or reoxidation damage of DNA, protein and lipid, damaging the integrity of cell structure and function, causing apoptosis or necrosis, tissue damage, cancer and aging. Oxidative stress is not only a part of liver dysfunction, but also the pathophysiological basis of liver injury.
Cell model is one of the effective methods to screen and evaluate antioxidants and study the mechanism of oxidative stress injury. Compared with animal model method, cell model can not truly simulate the internal environment, but it has the advantages of short cycle and low price.
Our Services
As a leader in the biotechnology industry, Creative Bioarray provides our customers with the establishment of hepatocyte oxidative stress model. We can induce human, mouse and chicken liver cells in many ways to make them suitable for a variety of research purposes.
Glucose oxidase (GO) is used to simulate the oxidative environment in order to establish a stable hepatocyte oxidative stress model. This method has the characteristics of stable H2O2.
The autophagy model of in vitro human hepatocyte line L02 is modeling with oxidant tert-butyl hydroperoxide (t-BHP). The model can be used to observe the role of mitochondrial oxidative stress and ROS related signaling pathway.
The establishment of chicken hepatocyte oxidative stress model is of great significance for revealing the mechanism of oxidative stress, researching and developing new antioxidant feed and reducing laying chicken liver injury.
In addition, we also provide drug administration experiment, pharmacopharmacology, efficacy evaluation and pharmacokinetic analysis of stress cell model to help customers study the mechanism of disease occurrence and development and seek new drugs for prevention and treatment.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you at the first time.
Reference
- Tell, G., Vascotto, C., & Tiribelli, C. (2013). Alterations in the redox state and liver damage: Hints from the EASL Basic School of Hepatology. Journal Of Hepatology, 58(2), 365-374.
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