Lipid Peroxidation Stress Assay
Lipid peroxidation is a recognized mechanism of cell injury in plants and animals. It is used as an indicator of oxidative stress in cells and tissues. Oxidative stress is related to natural aging process and a variety of disease states. It is caused by any of a variety of reactive oxygen species (ROS) that cannot be balanced by cells. The result is the destruction of one or more biomolecules, including DNA, RNA, proteins and lipids. The reaction between lipids and ROS (especially • oh) is a common mechanism of cellular oxidative damage. Free radicals attack unsaturated fatty acids, reduce membrane fluidity and affect cell physiological function.
Creative Bioarray provides our customers with lipid peroxidation detection services.
Lipid Peroxidation Stress Assay Services
Because lipid peroxidation is initiated by free radicals, unsaturated fatty acids form conjugated dienes in the initial stage of lipid oxidation caused by free radicals and some oxidation, and then form cyclic peroxides. The final main product is malondialdehyde (MDA).
Therefore, to judge the degree of lipid oxidative damage of the body or cells, on the one hand, it is necessary to detect the activity of antioxidant enzymes (SOD, cat, GPX) to judge the ability of drugs to scavenge oxygen free radicals, on the other hand, it is necessary to detect the level of lipid peroxidation product MDA to indirectly judge the severity of cell attack by free radicals. At the same time, the levels of SOD, cat, GPX and MDA were analyzed. The result analysis can more comprehensively evaluate the changes of oxidative stress level.
Creative Bioarray provides comprehensive lipid oxidative damage detection services. Our detection method is accurate, sensitive and fast. The specific service details are as follows
|
Service Name |
Assay Method |
Sensitivity & Detection Range |
Sample |
| Superoxide Dismutase (SOD) Activity Assay Service |
More stable / sensitive WST-8 chromogenic method |
3.13U/ml -200U/ml |
Serum, plasma, tissue / cell lysates and other biological fluids |
| Catalase Activity Assay Service |
The peroxidation function of catalase was directly used to determine catalase activity |
Low to 2 U/ml |
Biological samples such as serum, plasma, erythrocyte lysis products, tissue homogenate, cell lysate, etc |
| Lipid Peroxidation By-products Assay (for example MDA) |
Modified thiobarbituric acid (TBA) method |
1-100 µM/L ( MDA) |
Serum, plasma, urine, tissue / cell lysates and other biological fluids |
| Glutathione Peroxidase (GSH-Px) Assay |
The activity of H2O2 catalyzed by GSH PX enzyme was directly detected |
|
Serum, plasma, tissue / cell lysates and other biological fluids |
Other services
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you at the first time.
All services and products are for scientific use only, not for medical use！
