Mitochondrial Stress Test
These pathways promote the formation of cellular ROS, including mitochondrial electron transport chain (etc), inflammatory signal transduction and endoplasmic reticulum stress. Mitochondria and ROS play an important role in physiological and pathological processes. Different ROS, such as superoxide, hydrogen peroxide and peroxynitrite, stimulate different cellular signaling pathways and lead to different results according to their number and subcellular localization. Excessive ROS production may induce insulin resistance by activating the stress activated signal cascade or causing mitochondrial protein and DNA damage.
Creative Bioarray has now developed a variety of methods for detecting mitochondria and ROS, which is not only helpful for mitochondrial related scientific research, but also helpful for clinical diagnosis of mitochondrial diseases.
For several diseases, the therapeutic approaches include up regulation of mitochondrial biogenesis and improvement of mitochondrial function. Mitochondrial function and biogenesis can be assessed by:
- Mitochondrial stress test (OCR/ECAR)
- High content imaging of mitochondrial morphology and / or content
- Fatty acid oxidation analysis (of various fatty acid substrates β- Oxidation)
- Mitochondrial inner membrane permeability assay
- Measurement of mitochondrial membrane potential
Determining the effects of preclinical compounds on mitochondrial toxicity is an important tool to reduce the potential therapeutic risk.
Creative Bioarray provides comprehensive mitochondrial toxicity screening services using the most advanced equipment and testing methods. Mitochondrial toxicity was initially assessed by measuring mitochondrial oxygen consumption rate (OCR) and extracellular acidification rate (ecar) using industry standard analyzers.
Creative Bioarray offers our clients a ROS localization service in mitochondria. Through this service, we can specifically depict for our customers the different oxidative stress states of mitochondria in response to specific exogenous oxidative stimuli, promising to reveal the detailed mechanisms of intracellular oxidative stress.
For compounds found to inhibit oxidative phosphorylation, the toxicity mechanism was further improved by using advanced detection product lines to focus on the components of the electron transfer chain. Our analytical services are designed to measure the activity of complex I-IV and mitochondrial ATP synthase (also known as complex V) of etc, and are customized for each complex using different substrates and inhibitor groups.
Custom Cellular Models of Oxidative Stress Related Disease
Given the cost and time associated with in vivo disease models, the associated cell-based models can reduce some of the costs and allow rapid screening of potential lead candidates. With our industry standard instruments, state-of-the-art cell culture facilities, expertise, and access to our research compounds, dyes, and fluorescent probe directories, we can help develop custom cell-based disease models.
Using these models, our experts can characterize cell metabolic phenotypes and screen compounds that can improve functional and energy defects to help identify leading candidates related to therapeutic models.
Service Applications
- Mitochondrial toxicity assessment and preclinical compound de risk
- Measurement of mitochondrial biogenesis and functional improvement in different disease models
- Measurement of cellular oxidative stress or mitochondrial reactive oxygen species (ROS) production
- Studies on immune metabolism (metabolism and energetics of immune cells)
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
All services and products are for scientific use only, not for medical use！
