Mitochondrial Toxicity Assay
Mitochondria are known as the energy reservoir of the cell and play an important role in energy production, apoptosis, calcium signaling, regulation of cellular metabolism, and proliferation of heme and steroids.
Mitochondria have a unique structure with an outer membrane and an inner membrane. The inner membrane is highly convoluted and forms folds called cristae. Much of the supply of ATP, the cell's main energy source, is formed in these cristae. Five complexes (complexes I, II, III, IV and V) are located in the cristae and these complexes are involved in oxidative phosphorylation and ATP production.
The number of mitochondria in a cell depends on the type of tissue and its function. For example, due to increased energy demand, some organs are highly dependent on mitochondria, such as skeletal muscle, heart, brain and liver.
Mitochondria are common targets for drug toxicity. Creative Bioarray offers many different methods to assess mitochondrial toxicity in vitro.
Functional mitochondrial toxicity assays use Seahorse to detect the effects of compounds on oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) to assess cellular metabolism and mitochondrial function. Mitochondrial stress tests are used to understand cellular bioenergetics and mitochondrial toxicity mechanisms.
Permeabilization of the cell membrane leaves the mitochondrial membrane intact so that mitochondrial function can be studied without the need to isolate the mitochondria. By using complex-specific substrates and inhibitors, it is possible to identify the individual complexes involved in mitochondrial toxicity.
Evaluation of the ratio of mtDNA-encoded protein (COX-1, a subunit of complex IV) to nDNA-encoded protein (SDH-A, a subunit of complex II) using high content screening and fluorescently labeled antibodies
Using high-intensity imaging, mitochondrial mass and mitochondrial membrane potential can be detected using specific cellular dyes.
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All services and products are for scientific use only, not for medical use！
