Reactive Oxygen Species Assays

Fig. 1 Environmental factors maintain the balance of ROS in stem cells. (Chen et al., 2017)

Oxygen is electron reduced as part of normal metabolism, resulting in the formation of various ROS, including hydrogen peroxide (H₂O₂) and superoxide (O^2-). When uncontrolled oxidation puts pressure on biological systems, it will damage cellular macromolecules. The determination of ROS cannot identify the source of ROS production (i.e. normal state and disease state), but if the experimental model is under pressure, ROS may increase and the molecular composition may change.

Reactive oxygen species (ROS) such as superoxide and hydrogen peroxide are constantly produced in the metabolic process. The production of reactive oxygen species is usually balanced by the action of antioxidant enzymes and other redox molecules. However, excessive reactive oxygen species can lead to cell damage with damaged DNA, lipids and proteins.

Excess reactive oxygen species must be rapidly removed from cells through various antioxidant defense mechanisms. Cellular antioxidant enzymes and other redox molecules are used to balance reactive oxygen species produced in cells.

Creative Bioarray provides two general detection methods of reactive oxygen species: intracellular reactive oxygen species detection of intact cells and in vitro reactive oxygen species detection of cell lysates, tissue homogenates, blood or urine. In addition, we also provide specific quantitative analysis of hydrogen peroxide and nitric oxide.

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Fig. 2 Reactive Oxygen Species Assays

Hydrogen Peroxide Assays
Nitric Oxide Assays
In Vitro Nitric Oxide Assays
Intracellular Nitric Oxide Assay
Intracellular ROS Assay

Reactive oxygen species and reactive nitrogen can be measured directly by combining with fluorescent dyes. We provide two detection methods to generally detect ROS in various sample types:

Intracellular ROS assay for intact cultured cells
In vitro ROS / RNS assay of plasma, serum, urine, cell lysate or cell culture supernatant

Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you at the first time.

Reference

Chen, Y., Liu, H., Luo, X., Zhao, Z., Zou, Z., & Li, J. et al. (2017). The roles of reactive oxygen species (ROS) and autophagy in the survival and death of leukemia cells. Critical Reviews In Oncology/Hematology, 112, 21-30.

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