Fig. 1 Effect of antioxidants on bone cells at the molecular and cellular level in bone resorption. (Domazetovic et al., 2017)

Bone is one of the most important tissues in the human body. Oxidative stress injury of bone cells (such as chondrocytes and osteoblasts) is closely related to arthritis and osteonecrosis, which will bring serious pain and burden to patients. Oxidative stress is the sudden production of a large number of reactive oxygen species (ROS), which leads to oxidative damage due to abnormal metabolism, endogenous or exogenous stimulation, damage to the antioxidant defense system, destruction of oxygen free radical production and clearance, and accumulation of reactive oxygen species. Current studies have found that the pathogenesis of chronic inflammatory diseases and bone resorption-related diseases is related to oxidative stress caused by reactive oxygen species.

As an expert in the field of cellular stress, Creative Bioarray has established in Vitro osteoblast and chondrocyte oxidative stress models through a variety of stressors and can evaluate and screen antioxidative drugs based on these models. Our template is to help customers study the stress and/or pathological significance of the bone system, and provide important theoretical support for the identification of orthopedic-related diseases.

Service Cases

• Xanthine-Xanthine Oxidase Modeling Service for Osteoblast
  We established the oxidative stress model of human osteoblast-like cells by stimulating MG63 cells with superoxide anion produced by xanthine/xanthine oxidase (X / XO).
• H₂O₂ Oxidase Modeling Service for Osteoblast
• Fluoride Oxidase Modeling Service for Osteoblast
  Establishment of the oxidative stress model of osteoblast cells by NaF.
• Chondrocyte Biological Oxidative Stress Modeling Service
  We used Interleukin-1 β and tumor necrosis factor to induce oxidative stress and endoplasmic reticulum stress in human chondrocytes.
• Chondrocyte Chemical Oxidative Stress Modeling Service
  We used H₂O₂ oxidative stress and endoplasmic reticulum stress in rat and human chondrocytes.
• Osteoblast Oxidative Stress Level Evaluating Service
• Skeletal System Antioxidant Screening Service
  Based on the established oxidative stress model of the skeletal system, we can provide anti-ROS drug / antioxidant administration experiments, pharmacy pharmacology, efficacy evaluation, and pharmacokinetic analysis at the same time.
• Skeletal System Antioxidant Candidate Evaluating Service
  We will develop specific antioxidative stress programs and endoplasmic screening systems to help our clients assess the level of oxidative stress and the level of cell death.

Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.

Reference

1. Domazetovic, V., Marcucci, G., Iantomasi, T., Brandi, M. L., & Vincenzini, M. T. (2017). Oxidative stress in bone remodeling: role of antioxidants. Clinical cases in mineral and bone metabolism: the official journal of the Italian Society of Osteoporosis, Mineral Metabolism, and Skeletal Diseases, 14(2), 209–216.

Services

• Cell Level Oxidative Stress Assay Services
  • DNA & RNA Oxidative Stress Detection
  • Lipid Peroxidation Stress Assay
    • Lipid Peroxidation By-products Assay
    • Low Density Lipoprotein Oxidative Stress Assay Service
    • High Density Lipoprotein Oxidative Stress Assay Service
  • Protein Oxidation and Nitration Assays
  • Reactive Oxygen Species Assays
    • Hydrogen Peroxide Assays
    • Nitric Oxide Assays
    • Intracellular ROS Assay
    • In Vitro ROS & RNS Assay
  • Cell Antioxidant Assay Services
    • Antioxidant Capacity Assays Services
    • Antioxidant Enzyme Activity Assays
  • Oxidase & Peroxidase Activity Assay Service
  • Stressed Cell Migration Assay
  • Oxidative Stress Induced Apoptosis Assay Services
  • Stressed Cell Viability and Proliferation Assay
  • Immunohistochemistry Detection Service of ROS
  • Blood OS State Assay Services
  • Urine OS State Assay Services
• Oxidative Stress Cell Model Establishment Services
  • Establishment of Stress Model by Chemical Method
    • Extracellular H₂O₂ Exposure Model System
  • Hyperoxia Cell Oxidative Model System
  • Quality Evaluation Index of Oxidative Stress Cell Model
  • Digestive System Stress Model
    • Intestinal Epithelial Oxidative Stress Cell Model Construction Service
      • Intestinal Epithelial Cell Modeling Methods
      • Intestinal Epithelial Cell for Oxidative Stress Modeling
    • Hepatic Stellate Cell Oxidative Stress Model
      • Rat Hepatic Stellate Cells Oxidative Stress Model
      • Human Hepatic Stellate Cell Oxidative Stress Model
    • Hepatoma Cell Oxidative Stress Model
    • Hepatocyte Oxidative Stress Model
      • Human Hepatocyte Stress Model
      • Hepatocyte Mitochondrial Stress Autophagy Model
      • Chicken Hepatocyte Oxidative Stress Model
  • Reproductive System Oxidative Stress Model
    • Rat Ovarian Oxidative Stress Model
    • Mouse Ovarian Oxidative Stress Model
    • Human Ovarian Granulose Cells Oxidative Stress Model
    • Male Reproductive Cell Oxidative Stress Model
  • Endoplasmic Reticulum Stress Model
    • Liver Cancer Cells ER Stress Model
    • Rhinovirus Vector for ER Stress Modeling
    • Osteoblast ER Stress Model
    • Hepatocyte ER Stress Model
    • Cardiomyocyte ER Stress Model
  • Establishment of Stress Model by Biological Method
    • Porcine Circovirus Type 2 Induction
  • Skeletal System Oxidative Stress Modeling Service
    • Xanthine-Xanthine Oxidase Modeling Service for Osteoblast
    • H₂O₂ Oxidase Modeling Service for Osteoblast
    • Fluoride Oxidase Modeling Service for Osteoblast
    • Chondrocyte Biological Oxidative Stress Modeling Service
    • Chondrocyte Chemical Oxidative Stress
    • Osteoblast Oxidative Stress Level Evaluating Service
    • Skeletal System Antioxidant Screening Service
    • Skeletal System Antioxidant Candidate Evaluating Service
  • Circulatory System Oxidative Stress Modeling Service
    • Myocardial Oxidative Stress
      • Establishment of Myocardial Oxidative Stress Model
      • Cardiomyocyte Antioxidant Evaluation Service
      • Medicine Cardiac Toxicity Assessment Service
      • Gene-Level Stress Assessment of Cardiomyocyte
    • Umbilical Vein Endothelial Cell Oxidative Stress Modeling Service
    • Umbilical Vein Endothelial Cell Antioxidant Evaluation Service
  • Nervous System Oxidative Stress Modeling Service
    • Neural Cell Oxidative Stress Modeling Service
    • Neuroblastoma Oxidative Stress Modeling Service
    • Glioblastoma Oxidative Stress Modeling Service
    • Oxidation Neural Cell Gene Expression Analysis Service
    • Nervous System Antioxidant Candidate Assessment Service
    • Neural Cell Oxidation State Regulation Service
  • Skin System Oxidative Stress Modeling Service
    • Skin Fibroblast Oxidative Stress Service
      • Efficacy Evaluation Service for Anti-aging Substances
      • Regulation of Stress State in Skin Fibroblasts
    • Melanocyte Oxidative Stress Service
  • Animal Stress Modeling and Evaluation Services
    • Animal Oxidative Stress Modeling and Evaluation Services
      • Chicken Embryo Oxidative Stress Modeling Service
      • Pig Oxidative Stress Modeling Service
    • Animal Immune Stress Modeling and Evaluation Services
• Cell Oxidative Stress Assay Service Selection Guide
• Organelle Level Stress Assay
  • Mitochondrial Stress Test
    • Mitochondrial Oxidative Stress State Analysis
    • Mitochondrial Toxicity Assay
      • Functional Mitochondrial Toxicity Assay
      • Mitochondrial Respiratory Complex Assay
      • Mitochondrial Biogenesis Assay
      • HCS Mitochondrial Toxicity Assay
    • ROS Localization Services in Mitochondria
  • Endoplasmic Reticulum Stress Test
    • Continuous Analysis of ER Stress
    • Unfolded Protein Response Assay
    • Rapid Detection and Quantification Service of ER Stress Model
    • ER Stress Induction Platform
    • ER Redox State Monitoring
    • ER Calcium Distribution Measurement
    • Chemical and Genetic Tools to Assess ER Stress
  • Lipid Membrane Stress Test
• Non-oxidative Cellular Stress Assay
  • Hypoxia Stress Molecular Test Services
  • Heat Stress Molecular Test Services